Characterization of Exogenous Sequence Fragments in Extracellular Vesicles from Human

Extracellular vesicles (EVs) play crucial role in mediating intercellular communication. Small RNA is an important component EVs. However, the proportion of small sequencing (smRNA-seq) reads EVs mapped to human genome much lower than that cells, suggesting existence many nonhuman sequences there no systematic study on EV fragments unmapped genome. Herein, using smRNA-seq data, landscape exogenous cargoes portrayed. The results show distribution sequence 1838 samples; average 21.82% are genome, and 12.33% collected reference sequences. Furthermore, plasma samples lowest, while cell line samples, it higher, mainly from animals, bacteria, or contaminants. Exogenous plants food, bacteria gut microbiota. Virus-derived reflect high prevalence viruses population, such as herpesvirus hepatitis virus. This provides first implies diverse sources body.